Machine-perfused donor kidneys as a source of human renal endothelial cells

We describe a new and widely accessible approach to obtain human primary renal endothelial cells in standardized fashion, by isolating from the perfusate of machine-perfused donor kidneys. Characterization showed mixed population originating different compartments kidney. As proof principle, we demonstrated possible diagnostic application an endothelium-specific cross-match. Next transplantation, foresee further applications field research.